Homepage of Remus Th. Dame, PhD

Vrije Universiteit, Amsterdam

Last modified: 2/3/10

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PhD positions

I am currently looking for motivated and talented individuals to join my new lab in the Cell Observatory at Leiden University.

If you have a training in biophysics, biochemistry or molecular biology and strong interest in a multi-disciplinary approach to quantitative biology, please apply by sending a letter of motivation, your cv and the names of at least two referees.

Please check my web site at Leiden University for further details.

The organization of bacterial chromatin and its interplay with transcription
Bacterial chromosomal DNA is not confined to an envelope-enclosed organelle such as the nucleus in eukaryotes, yet the volume it occupies has to be reduced below that of the cell. An unconstrained chromosomal DNA molecule of 1.6 mm as found in E. coli would form a random coil with a volume of ~200 um3. Due to the action of a number of factors a DNA molecule of this size can nevertheless be fit into a cell that is only 2 um long and 1 um wide. These factors include macromolecular crowding (yielding a phase separation between cytoplasm and DNA) and DNA supercoiling.

Our primary interest is the role that a group of small architectural proteins (called nucleoid-associated proteins - NAP's) plays in organizing and compacting the bacterial chromosomal DNA. These proteins can be divided into two main groups based on their mode of action: bridgers and benders.

DNA bridging proteins (H-NS, StpA, Lrp, SMC proteins, …) usually have a multimeric structure with multiple DNA binding domains, providing a means to interact with two DNA duplexes simultaneously. We have investigated the activity of H-NS like proteins as organizers of bacterial chromatin and the structural basis of repression mechanisms. SMC proteins are much larger in size when compared to the other NAP's and presumable act by enclosing multiple DNA duplexes rather than by directly interacting with the DNA.

DNA bending proteins (HU, IHF, Fis, ... ) bind DNA either at specific or non-specific sites and in doing so distort it. The bending by these proteins amounts 50-90 (in the case of Fis) and up to ~180 degrees (in the case of HU and IHF). Both bridging and bending yield compaction and aid in the organization of the chromosomal DNA. Interestingly both HU and Fis have an alternative 'binding mode' in which they form filaments around DNA.

Most of these proteins do not just play a role as organizers of bacterial chromatin, but also act as regulators of transcription. For instance, IHF and Fis act as direct activators of transcription by binding at specific sites upstream of the promoter. Similarly, H-NS directly represses transcription by binding preferentially at A/T-rich/flexible regions found close to H-NS sensitive promoters. Besides such direct effects, transcription of a large set of genes is regulated indirectly by opposing the effects of a second NAP. For instance, many genes repressed by H-NS, are specifically de-repressed by IHF, HU or Fis. The latter set of proteins is expressed differently during different phases of growth, which allows different subsets of genes to be de-repressed. Since these proteins act both at specific sites and non-specific sites, their differences in expression probably also give rise to differences in global genome organization that indirectly affect transcription levels from a substantial number of additional genes.