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Publications
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Molecular Biophysics Group
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54 |
I.L. Ivanovska, R. Miranda, J.L. Carrascosa, C.F. Schmidt*, G.J.L. Wuite* Discrete fracture patterns of virus shells reveal mechanical building blocks (submitted) |
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53 |
A. Evilevitch, I.L. Ivanovska, W.H. Roos, M. Jeembaeva, B. Jönsson, G.J.L. Wuite Effects of salts on internal DNA pressure and mechanical stability of phages (submitted) |
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52 |
P. Gross, G. Farge, H. Geertsma, T. Hoekstra, E.J.G. Peterman, G.J.L. Wuite Generating single-stranded DNA templates for optical tweezers-experiments (submitted) |
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51 |
W. H. Roos, R. Bruinsma G. J. L. Wuite Physical Virology: Self-Assembly and Mechanics Nature Physics (in press) |
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50 |
M. Baclayon, W.H. Roos, G.J.L. Wuite Sampling protein form and function using Atomic Force Microscope Mol Cell Proteomics 9(8):1678-88 (2010) |
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49 |
W.H. Roos, M.M. Gibbons, A. Arkhipov, C. Uetrecht, N.R. Watts, P.T. Wingfield, A.C. Steven, A.J.R. Heck, K. Schulten, W.S. Klug, G.J.L. Wuite Deforming nanostructures: how continuum elastic models, molecular dynamics simulations and experiments coalesce at the nanoscale Biophysical Journal 98(9):1902-1910 (2010) Featuring as Research Highlight |
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48 |
M. Zaremba, A. Owsicka, G. Tamulaitis, G. Sasnauskas, L. Shlyakhtenko, A. Lushnikov, Y. Lyubchenko, N. Laurens, B. v/d Broek, G.J.L. Wuite, V. Siksnys DNA synapsis through the transient tetramerization triggers the cleavage by Ecl18kI restriction enzyme Nucleic Acids Research (in press) Article features as outstanding paper |
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47 |
J. van Mameren, G.J.L. Wuite, I. Heller Introduction to optical tweezers: background, system designs, and commercial solutions Chapter to be published in Methods in Molecular Biology: Single-Molecule techniques, Wuite, G.J.L. & Peterman, E.J.G. (Eds.), Humana Press |
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46 |
M. Baclayon, G. J. L. Wuite, W. H. Roos Imaging and manipulation of single viruses by atomic force microscopy Soft Matter (in press) doi: 10.1039/b923992h + cover |
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45 |
G. K. Shoemaker, E. van Duijn, S.E. Crawford, C. Uetrecht, M. Salumbides-Baclayon, W.H. Roos, G.J.L. Wuite, M.K. Estes, B.V. Venkataram Prasad, A.J.R. Heck Norwalk virus assembly and stability monitored by mass spectrometry Mol Cell Proteomics 9(8):1742-51 (2010) |
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44 |
R.J. Kroeze, M.N. Helder, W.H. Roos, G.J.L. Wuite, V. Everts, R.A. Bank, T.H. Smit The effect of ethylene oxide, glow discharge and electron beam on the surface characteristics of poly(l-lactide-co-caprolactone) and the corresponding cellular response of adipose stem cells Acta Biomaterialia 6(6):2060-2065 (2010) doi:10.1016/j.actbio.2009.11.022 |
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43 |
W.H. Roos and G.J.L. Wuite Mechanical properties of viruses In Emerging Topics in Physical Virology, Stockley, P. & Twarock, R. (Eds.), London: Imperial College Press (2010) |
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42 |
B. van den Broek, M.C. Noom, J. van Mameren, C. Battle, F.C. MacKintosh, G.J.L. Wuite Visualizing the formation and collapse of DNA toroids Biophysical Journal 98(9):1902-10 (2010) |
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41 |
R Metzler, B van den Broek, G.J.L. Wuite, M.A. Lomholt Role of DNA conformation in gene regulation In Biophysics of DNA-protein interactions: From single molecules to biological systems, Williams, M & Maher, J (Eds.), Springer (2010) |
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40 |
P. Gross, G. Farge, E.J.G. Peterman, G.J.L. Wuite Combining optical tweezers, single molecule fluorescence microscopy and microfluidics for studies of DNA-proteins interactions In Methods in Enzymology: Single Molecule Methods, Walter, N (Ed.), Elsevier (2010) |
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39 |
J. van Mameren, P. Gross, G. Farge, P. Hooijman, M. Modesti, M. Falkenberg, E.J.G Peterman*, G.J.L. Wuite* Unraveling the structure of DNA during overstretching using multicolor, single-molecule fluorescence imaging Proc. Natl. Acad. Sci. U. S. A. 106:18231-18236 (2009) See also: Commentary by Williams et al. in PNAS & in this issue highlight |
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38 |
Metzler R, Koren T, van den Broek B, Wuite GJL, Lomholt MA And did he search for you, and could not find you? Journal of Physics A 42(43):434005 (2009) |
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37 |
P.A. Wiggins, R.T. Dame, M.C. Noom, G.J.L. Wuite Protein mediated bridging motifs: a key mechanism in biopolymer organization Biophysical Journal 97(7): 1997-2003 (2009) Selected as Featured article by Biophys Journal |
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36 |
C. Battle, B. van den Broek, M.C. Noom, J. van Mameren, G.J.L. Wuite, F.C. MacKintosh Unraveling DNA tori under tension Physical Review E 80(3): 031917 Part 1 (2009) |
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35 |
A. Arkhipov, W.H. Roos, G.J.L. Wuite, K. Schulten Elucidating the mechanism behind irreversible deformation of viral capsids Biophysical Journal 97(7): 2061-2069 (2009) |
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34 |
W.H. Roos, K. Radtke, E. Kniesmeijer, H. Geertsema, B. Sodeik, G.J.L. Wuite Scaffold expulsion and genome packaging trigger stabilization of Herpes Simplex Virus capsids Proc. Natl. Acad. Sci. U. S. A. 106:9673-9678 (2009) See also: Science Daily & Twitter report |
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33 |
N. Laurens, S. Bellamy, A. Harms, Y. Kovacheva, S. Halford, G.J.L. Wuite Dissecting protein-induced DNA looping dynamics in real time Nucleic Acids Research (2009) doi: 10.1093/nar/gkp570 |
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32 |
M. Lomholt, B. van den Broek, S.-M. Kalisch, G.J.L. Wuite, R. Metzler Facilitated diffusion with DNA coiling Proc. Natl. Acad. Sci. U. S. A. 106:8204-8208 (2009) |
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31 |
J. van Mameren, M. Modesti R. Kanaar, C. Wyman, E.J.G. Peterman, G.J.L. Wuite Counting RAD51 proteins disassembling from nucleoprotein filaments under tension Nature 457, 745 - 748 (2009), doi: 10.1038/nature07581 See also: NRC (premier Dutch newspaper), 13 Dec. 2008 |
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30 |
W.H. Roos, G.J.L. Wuite Nanoindentation studies reveal material properties of viruses Advanced Materials 21 (10-11):1187-1192 (2009) doi: 10.1002/adma.200801709 |
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29 |
D. Millo, A. Ranieri, P. Gross, H.K. Ly, M. Borsari, P. Hildebrandt, G.J.L. Wuite, C. Gooijer, G. van der Zwan The electrochemical response of cytochrome c immobilized on smooth and roughened silver and gold surfaces chemically modified with 11-mercaptounodecanoic acid Journal of Physical Chemistry 113 (7): 2861–2866 (2009) |
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28 |
C.I. Zoldesi, I.L. Ivanovska, C. Quilliet, G.J.L. Wuite, A. Imhof Elastic properties of hollow colloidal particles Physical Review E 78: 051401 (2008) |
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27 |
B. van den Broek, M.A. Lomholt, S-M.J. Kalisch, R. Metzler, G.J.L. Wuite How DNA coiling enhances target localization by proteins Proc. Natl. Acad. Sci. U. S. A. 105 (41):15738-15742 (2008) See also Highlight: Nature Structural & Molecular Biology 15, 1137 (2008) NRC (premier Dutch newspaper), 18 Oct. 2008 |
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26 |
J. van Mameren, E.J.G. Peterman, and G.J.L. Wuite See me, feel me: methods to concurrently visualize and manipulate single DNA molecules and associated proteins Nucleic Acids Research 36:4381-4389 (2008) |
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25 |
C. Uetrecht, C. Versluis, N. Watts, W.H. Roos, G.J.L. Wuite, P. Wingfield, A.C. Steven, A.J.R. Heck Structure, Stability and Shape of Hepatitis B Virus Capsids Proc. Natl. Acad. Sci. U. S. A. 105(27): 9216-9220 (2008) |
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24 |
M.C. Noom, B. van den Broek, J. van Mameren, G.J.L. Wuite Visualizing single DNA-bound proteins using DNA as a scanning probe Nature Methods 4, 1031 - 1036 (2007) + cover See also Highlight; Lab on a Chip 8, 195 (2008) |
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23 |
M.C. Noom, W.W. Navarre, T. Oshima, G.J.L. Wuite, R.T. Dame H-NS promotes looped domain formation in the bacterial chromosome Current Biology 17(21): R913-R914 (2007) |
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22 |
G.J.L. Wuite*, I.L. Ivanovska*, B. Jönsson, A. Evilevitch Internal DNA pressure modifies stability of wild-type phage Proc. Natl. Acad. Sci. U. S. A. 104:9603-9608 (2007) |
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21 |
W. Roos, I.L. Ivanovska, A. Evilevitch, G.J.L. Wuite Viral capsids: mechanical characteristics, genome packaging and delivery mechanisms Cellular and Molecular Life Sciences 64: 1484-1497 (2007) doi:10.1007/s00018-007-6451-1 |
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20 |
M. Modesti, D. Ristic, T. v.d. Heijden, C. Dekker, J. v Mameren, E.J.G. Peterman, G.J.L. Wuite, R. Kanaar, C. Wyman Fluorescent human RAD51 reveals multiple nucleation sites and filament segments tightly associated along a single DNA molecule Structure 15:599-609 (2007) |
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19 |
R.T. Dame, M.C. Noom, G.J.L. Wuite Bacterial chromatin organization by H-NS protein unraveled using a dual DNA assay Nature 444:387-390 (2006) See also exclusive review in BioEssays 29 (3):212 - 216 (2007) See also conference report in Nature Chemical Biology 3(4):193–197 (2007) Extensive media coverage in the Netherlands |
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18 |
W.S. Klug, R.F. Bruinsma, J.P Michel, C.M. Knobler, I.L. Ivanovska, C.F. Schmidt, G.J.L. Wuite Failure of Viral Shells Physical Review Letters 97:228101 (2006) See also News and Views; Nature Physics 3, 19 (2007); doi:10.1038/nphys500 |
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17 |
J. van Mameren, M. Modesti, R. Kanaar, C. Wyman, G.J.L. Wuite, E.J.G. Peterman Dissecting elastic heterogeneity along DNA molecules coated partly with Rad51 using concurrent fluorescence microscopy and optical tweezers Biophysical Journal 91(8):L78-L80 (2006) doi:10.1529/biophysj.106.089466 |
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16 |
M.S. Luijsterburg, M.C. Noom, G.J.L. Wuite, R.T. Dame The architectural role of nucleoid-associated proteins in the organization of bacterial chromatin: A molecular perspective Journal of Structural Biology 165, 262-272 (2006) doi:10.1016/j.jsb.2006.05.006 |
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15 |
J.P. Michel, I.L. Ivanovska, M.M. Gibbons, W.S. Klug, C.M. Knobler, G.J.L. Wuite, C.F. Schmidt Nanoindentation studies of full and empty viral capsids and the effects of capsid protein mutations on elasticity and strength Proc. Natl. Acad. Sci. U. S. A. 103:6184-6189 (2006) |
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14 |
B. van den Broek, F. Vanzi, D. Normanno, F.S. Pavone, G.J.L. Wuite Real-time observation of DNA looping dynamics of type IIE restriction enzymes NaeI and NarI Nucleic Acids Research 34(1) 167-174 (2006) |
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13 |
K.C. Vermeulen, G.J.L. Wuite, G.J.M. Stienen, and C.F. Schmidt Optical trap stiffness in the presence and absence of spherical aberrations Appl. Opt. 45: 1812-1819 (2006) |
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12 |
K.C. Vermeulen, J. van Mameren, G.J.M. Stienen, E.J.G. Peterman, G.J.L. Wuite, C.F. Schmidt Calibrating bead displacements in optical tweezers using acousto-optic deflectors Review of Scientific Instruments 77:013704 (2006) |
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11 |
S.R. Leliveld, R.T. Dame, G.J.L. Wuite, Lothar Stitz, C. Korth Molecular basis of genetic Creutzfeldt Jakob disease linked to expansion of the prion protein octarepeat domain J. Biol. Chem. 281 (6):3268-3275 (2006) |
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10 |
B. van den Broek, M.C. Noom, and G.J.L. Wuite DNA-tension dependence of restriction enzyme activity reveals mechanochemical properties of the reaction pathway Nucleic Acids Research 33(8):2676-2684 (2005) |
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9 |
R.T. Dame, J. van Mameren, M.S. Luijsterburg, M.E. Mysiak,A. Janiijevi, G. Pazdzior, Simulation-based estimation of protein-induced DNA bending angles by scanning force microscopy Nucleic Acids Research 33(7):e68 (2005) See also: Online supporting software |
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8 |
R.T. Dame, M.S. Luijsterburg, E. Krin, P.N. Bertin, R. Wagner, G.J.L. Wuite DNA bridging: a property shared among H-NS-like proteins J. Bacteriol. 187:1845-8 (2005) |
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7 |
I.L.Ivanovska, P.J. de Pablo, B. Ibarra, G. Sgalari, F.C. MacKintosh, J.L.Carrascosa, C.F. Schmidt, G.J.L. Wuite Bacteriophage capsids: tough nano-shells with complex elastic properties Proc. Natl. Acad. Sci. U. S. A. 101:7600-7605 (2004) |
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6 |
R.T.Dame and G.J.L. Wuite On the role of H-NS in the organization of bacterial chromatin. From bulk to single molecules and back Biophysical Journal 85:4146-4148 (2003) |
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5 |
N.Forde, D. Izhaky, G. Woodcock, G.J.L. Wuite, C. Bustamante Using mechanical force to probe the mechanism of pausing and arrest during continuous elongation by E. coli RNA polymerase Proc. Natl. Acad. Sci. U. S. A. 99:11682-11687(2002) |
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4 |
G.J.L. Wuite, S.B. Smith, M. Young, D. Keller, C. Bustamante Single Molecule Studies of the Effect of Template Tension on T7 DNA Polymerase Activity |
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3 |
G.J.L. Wuite*, J. Davenport*, R. Landick, C. Bustamante See a movie of this work on RNAp: a Single RNAP Molecule transcribing |
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2 |
C. Bustamante, J. Macosko, G.J.L. Wuite Grabbing the Cat by the Tail: Manipulating Molecules One by One |
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1 |
G.J.L. Wuite, J. Davenport, A. Rappaport, C. Bustamante An Integrated Laser Trap/Flow Control Video Microscope for the Study of Single Biomolecules |
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*Equal contributions |
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Proceedings, Patents & Other |
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G.J.L. Wuite |
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W.H. Roos, C. Uetrecht, N. Watts, P. Wingfield, A.C. Steven, A.J.R. Heck, J.L. Carrascosa, C. F. Schmidt, G.J.L. Wuite Force Spectroscopy reveals mechanical structure of capsids Proceedings of the European Microscopy Congress 3:215-216 (2008) |
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G.J.L. Wuite |
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M.C. Noom, R.T. Dame, G.J.L. Wuite Het bacterieel chromosoom ontrafeld met een viervoudig optisch pincet Ned. Tijdschr. v. Nat. 73 (2):46-48 (2007) |
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G.J.L. Wuite Single Molecule Studies of RNA and DNA Polymerases using Laser Tweezers PhD Thesis, January 6th (2000) Advisors: Prof. J. Greve & Prof. C. Bustamante |
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M. Bennink and G.J.L. Wuite |
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