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Prof. dr. Gijs Wuite
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Physics of Life Processes Group
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My group is part of the Physics of Living Systems Section in the faculty of Sciences at the VU University Amsterdam. The research in the group focuses on exploring DNA-proteins interactions and biophysical/biomechanical properties of viral capsids & cells. The aim is to work with increasingly more complex assemblies of proteins in order to investigate the emergent properties from these systems. This approach bridges experimental systems biology and single-molecule manipulation techniques. We use a variety of techniques such as optical tweezers, AFM and single-molecule fluorescence as well as combinations of these techniques. The data obtained are related to biochemical studies and used for theoretical modeling. |
Selected Publications
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News
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Nov 12: Our FOM program was approved (see open positions) Okt 12: Starting with a new projectruimte grant on viruses (see open positions) Aug 11: Our Nature Physics on DNA mechanics is published Aug 10: I obtained a ERC starting grant May 10: Movie of my research @ Volkskrant.nl & Movie of PhD student Niels Laurens in my group in a Popular Talk show “De Wereld Draait Door”, Jan 2010 Apr 10: Our Human Frontier Grant has been approved Feb 10: Our program in the FES grant HTS&M has been granted Jan 09: Alumni Postdoc Remus Dame starts his own group at Leiden University Dec 08: My 1.3 MEuro VICI grant got approved Dec 08: Two articles about our research appeared in the major Dutch newspaper, the NRC Nov 08: Selected for The Young Academy, part of the Royal Dutch Academy of Sciences, its 80 members are selected for scientific achievements and having received PhD <10 years ago |
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G. Farge, N. Laurens, O.D. Broekmans, S.M.J.L. van den Wildenberg, L.C.M. Dekker, M. Gaspari, C.M. Gustafsson, E.J.G. Peterman, M. Falkenberg, G.J.L. Wuite Protein sliding and DNA denaturation are essential for DNA organization by human mitochondrial transcription factor A Nature Communications 3:1013 (2012) |
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W.H. Roos, I. Gertsman, E.R. May, C.L. Brooks, J.E. Johnson, G.J.L. Wuite Mechanics of bacteriophage maturation Proc. Natl. Acad. Sci. U. S. A. 109(7):2342-2347 (2012) |
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P. Gross, N. Laurens, L.B. Oddershede, U. Bockelmann, E.J.G. Peterman, G.J.L. Wuite Quantifying how DNA stretches, melts and changes twist under tension Nature Physics 7(9):731-736 (2011) |
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W. H. Roos, R. Bruinsma G. J. L. Wuite Physical Virology Nature Physics 6(10):733-743 (2010) |
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J. van Mameren, P. Gross, G. Farge, P. Hooijman, M. Modesti, M. Falkenberg, E.J.G Peterman*, G.J.L. Wuite* Unraveling the structure of DNA during overstretching using multicolor, single-molecule fluorescence imaging Proc. Natl. Acad. Sci. U. S. A. 106:18231-18236 (2009) |
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W.H. Roos, K. Radtke, E. Kniesmeijer, H. Geertsema, B. Sodeik, G.J.L. Wuite Scaffold expulsion and genome packaging trigger stabilization of Herpes Simplex Virus capsids Proc. Natl. Acad. Sci. U. S. A. 106:9673-9678 (2009) |
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J. van Mameren, M. Modesti R. Kanaar, C. Wyman, E.J.G. Peterman, G.J.L. Wuite Counting RAD51 proteins disassembling from nucleoprotein filaments under tension Nature 457, 745 - 748 (2009) |
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B. van den Broek, M.A. Lomholt, S-M.J. Kalisch, R. Metzler, G.J.L. Wuite How DNA coiling enhances target localization by proteins Proc. Natl. Acad. Sci. U. S. A. 105 (41):15738-15742 (2008) See also Highlight: Nature Structural & Molecular Biology 15, 1137 (2008) |
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M.C. Noom, B van den Broek, J. van Mameren, G.J.L. Wuite Visualizing single DNA-bound proteins using DNA as a scanning probe Nature Methods 4, 1031-1036 (2007) Cover by Maarten Noom |
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G.J.L. Wuite*, I.L. Ivanovska*, B. Jönsson, A. Evilevitch Internal DNA pressure modifies stability of wild-type phage Proc. Natl. Acad. Sci. U. S. A. 104: 9603-9608 (2007) |
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R.T. Dame, M.C. Noom, G.J.L. Wuite Bacterial chromatin organization by H-NS protein unraveled using a dual DNA assay Nature 444:387-390 (2006) See also exclusive review in BioEssays 29 (3), 212 - 216 (2007) |
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W.S. Klug, R.F. Bruinsma, J.P Michel, C.M. Knobler, I.L. Ivanovska, C.F. Schmidt, G.J.L. Wuite Failure of Viral Shells Physical Review Letters 97:228101 (2006) See also News and Views; Nature Physics 3, 19 (2007) |
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I.L.Ivanovska, P.J. de Pablo, B. Ibarra, G. Sgalari, F.C. MacKintosh, J.L.Carrascosa, C.F. Schmidt, G.J.L. Wuite Bacteriophage capsids: tough nano-shells with complex elastic properties Proc. Natl. Acad. Sci. U. S. A. 101:7600-7605 (2004) |
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N.Forde, D. Izhaky, G. Woodcock, G.J.L. Wuite, C. Bustamante Using mechanical force to probe the mechanism of pausing and arrest during continuous elongation by E. coli RNA polymerase Proc. Natl. Acad. Sci. U. S. A. 99:11682-11687(2002) |
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G.J.L. Wuite, S.B. Smith, M. Young, D. Keller, C. Bustamante Single Molecule Studies of the Effect of Template Tension on T7 DNA Polymerase Activity |
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G.J.L. Wuite*, J. Davenport*, R. Landick, C. Bustamante |
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*Equal contributions |