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Sept 09: I’m appointed as full Professor of Physics of Life Processes Sept 09: Onno Broekmans, Iddo Heller, Andreas Biebricher & Sandro Perrone join the group Apr 09: Tjalle Hoekstra and Marian Salambides join the group Feb 09: Our new Nature paper is online. Jan 09: Alumni Postdoc Remus Dame starts his own group at Leiden University Dec 08: My 1.3 MEuro VICI grant gets approved (see open positions) Dec 08: Two articles about our research appeared in the major Dutch newspaper, the NRC Dec 08: Geraldine Farge joins the group Nov 08: Selected for The Young Academy, part of the Royal Dutch Academy of Sciences, its 80 members are selected for scientific achievements and having received PhD <10 years ago May 08: My CW Echo grant gets approved |
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P.I. Prof. Dr. Gijs Wuite
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Molecular Biophysics Group
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My group is associated with the Physics of Complex Systems Section in the faculty of Sciences at the VU University Amsterdam. The research in the group focuses on exploring DNA-proteins interactions and biophysical/biomechanical properties of viral capsids & cells. The aim is to work with increasingly more complex assemblies of proteins in order to investigate the emergent properties from these systems. This approach bridges experimental systems biology and single-molecule manipulation techniques. We use a variety of techniques such as optical tweezers, AFM and single-molecule fluorescence as well as combinations of these techniques. The data obtained are related to biochemical studies and used for theoretical modeling. |
Selected Publications
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News
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J. van Mameren, P. Gross, G. Farge, P. Hooijman, M. Modesti, M. Falkenberg, E.J.G Peterman*, G.J.L. Wuite* Unraveling the structure of DNA during overstretching using multicolor, single-molecule fluorescence imaging Proc. Natl. Acad. Sci. U. S. A. (in press) |
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W.H. Roos, K. Radtke, E. Kniesmeijer, H. Geertsema, B. Sodeik, G.J.L. Wuite Scaffold expulsion and genome packaging trigger stabilization of Herpes Simplex Virus capsids Proc. Natl. Acad. Sci. U. S. A. 106:9673-9678 (2009) |
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10 |
J. van Mameren, M. Modesti R. Kanaar, C. Wyman, E.J.G. Peterman, G.J.L. Wuite Counting RAD51 proteins disassembling from nucleoprotein filaments under tension Nature 457, 745 - 748 (2009) |
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B. van den Broek, M.A. Lomholt, S-M.J. Kalisch, R. Metzler, G.J.L. Wuite How DNA coiling enhances target localization by proteins Proc. Natl. Acad. Sci. U. S. A. 105 (41):15738-15742 (2008) See also Highlight: Nature Structural & Molecular Biology 15, 1137 (2008) |
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M.C. Noom, B van den Broek, J. van Mameren, G.J.L. Wuite Visualizing single DNA-bound proteins using DNA as a scanning probe Nature Methods 4, 1031-1036 (2007) Cover by Maarten Noom |
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G.J.L. Wuite*, I.L. Ivanovska*, B. Jönsson, A. Evilevitch Internal DNA pressure modifies stability of wild-type phage Proc. Natl. Acad. Sci. U. S. A. 104: 9603-9608 (2007) |
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R.T. Dame, M.C. Noom, G.J.L. Wuite Bacterial chromatin organization by H-NS protein unraveled using a dual DNA assay Nature 444:387-390 (2006) See also exclusive review in BioEssays 29 (3), 212 - 216 (2007) |
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W.S. Klug, R.F. Bruinsma, J.P Michel, C.M. Knobler, I.L. Ivanovska, C.F. Schmidt, G.J.L. Wuite Failure of Viral Shells Physical Review Letters 97:228101 (2006) See also News and Views; Nature Physics 3, 19 (2007) |
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I.L.Ivanovska, P.J. de Pablo, B. Ibarra, G. Sgalari, F.C. MacKintosh, J.L.Carrascosa, C.F. Schmidt, G.J.L. Wuite Bacteriophage capsids: tough nano-shells with complex elastic properties Proc. Natl. Acad. Sci. U. S. A. 101:7600-7605 (2004) |
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N.Forde, D. Izhaky, G. Woodcock, G.J.L. Wuite, C. Bustamante Using mechanical force to probe the mechanism of pausing and arrest during continuous elongation by E. coli RNA polymerase Proc. Natl. Acad. Sci. U. S. A. 99:11682-11687(2002) |
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G.J.L. Wuite, S.B. Smith, M. Young, D. Keller, C. Bustamante Single Molecule Studies of the Effect of Template Tension on T7 DNA Polymerase Activity |
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G.J.L. Wuite*, J. Davenport*, R. Landick, C. Bustamante |
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*Equal contributions |