Fluorescence spectra
Fluorescence spectra that are recorded you can e-mail to yourself. The text file that you will then receive contains in the first column the wavelengths of the fluorescence spectrum measured, and in the second column showed the relative intensities of the fluorescence (in arbitrary units). Processing of this data can be done in any data analysis software (e.g. Origin, Igor, R or even Excel is you wish).
A simple fitting application is also available (for advanced eror analysis you will need to use data analysis software). The fitting application allows you to fit the spectra with Gaussian functions to discriminate between the two photosystems, and one can derive the peak positions, maxima values and the full-width-at-half-maximum (FWHM) of these Gaussians.
Time spectra
Similarly, time spectra that are recorded you can e-mail to yourself. The text file is build up similarly as well: columns with time and total intensity for the two wavelength regions selected. Again advanced error analysis will require data analysis software.
The simple fitting application available always you to perform exponential fits on the time traces, and one can determine tau, the amplitude and steady-state value of fluorescence, and the mean-squared error (mse). The residue of the exponential fit is also given.
