In our lab we employ a range of techniques, to study dynamics events in cells and proteins:

Ultrafast laserspectroscopy:

  • Pump-probe spectroscopy allows the detection of absorption changes over a time window from tens of femtoseconds to nanoseconds, over wavelength regions from the UV to the midinfrared.

  • Time-resolved fluorescence measurements on a (sub)picosecond to nanosecond timescale with the use of a multiwavelength streakcamera system.

  • Photon-echo experiments on a femto- to picosecond timescale allow the study of electronic dephasing processes.

Steady-state techniques:

  • Absorption, linear dichroism, circular dichroism, (polarized) fluorescence, fluorescence line narrowing and Stark spectroscopy.

Microscopy

  • Confocal microscope

  • Multiphoton microscope

Experiments are often performed at cryogenic temperatures, down to 4.2 K. Fundamental research in this field requires highly purified, sometimes genetically engineered, biological material with
well-defined physical, chemical and biological properties. Thus, a multi-disciplinary approach involving several disciplines (physics, chemistry, biology, mathematics, genetics) is absolutely essential for competitive research in this branch of science.

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